The changing science of HIV epidemiology in the United States. Cohort profile: the Women’s Interagency HIV Study (WIHS). Two subsets of memory T lymphocytes with distinct homing potentials and effector functions. Sallusto, F., Lenig, D., Förster, R., Lipp, M. High frequency of shared clonotypes in human T cell receptor repertoires. T cell fate and clonality inference from single-cell transcriptomes. Mechanisms for T cell receptor triggering. Adoptive transfer of CD4 + T cells reactive to modified low-density lipoprotein aggravates atherosclerosis. T-cells specific for a self-peptide of ApoB-100 exacerbate aortic atheroma in murine atherosclerosis. Depletion of FOXP3 + regulatory T cells promotes hypercholesterolemia and atherosclerosis. Role of naturally occurring CD4 +CD25 + regulatory T cells in experimental atherosclerosis. Natural regulatory T cells control the development of atherosclerosis in mice. Atherosclerosis: a chronic inflammatory disease with an autoimmune component. T cell subsets and functions in atherosclerosis. Pathogenic autoimmunity in atherosclerosis evolves from initially protective apolipoprotein B 100–reactive CD4 + T-regulatory cells. Regulatory CD4 + T cells recognize major histocompatibility complex class II molecule–restricted peptide epitopes of apolipoprotein B. Immunity and inflammation in atherosclerosis. Mouse studies showed that such switched cells promote atherosclerosis. These findings suggest that APOB-specific regulatory T cells may switch to a more memory-like phenotype in women with atherosclerosis. T cell receptor sequencing of tetramer-positive cells showed clonal expansion and V and J segment usage similar to those found in regulatory T cells.

Single-cell RNA sequencing showed that transcriptomes of tetramer-positive cells were between regulatory and memory T cells in healthy women and moved closer to memory T cells in women with sCVD. Here, we produce, validate and use an MHC-II tetramer, DRB1*07:01 APOB-p18, to sort APOB-p18-specific CD4 T cells from peripheral blood mononuclear cell samples from eight DRB1*07:01 + women with and without subclinical cardiovascular disease (sCVD). Major histocompatibility complex class II (MHC-II) tetramers can be used to isolate antigen-specific CD4 T cells by flow sorting. Nature Cardiovascular Research volume 1, pages 462–475 ( 2022) Cite this articleĪtherosclerosis is accompanied by a CD4 T cell response to apolipoprotein B (APOB). Single cell transcriptomics and TCR reconstruction reveal CD4 T cell response to MHC-II-restricted APOB epitope in human cardiovascular disease

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